FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11206548
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA22400.0112.352941
GAGCAAA176250.070.030421
GCAAAAG175300.070.015543
AGCAAAA196950.062.469882
GCAGGGT93750.056.142989
CAAAAGC250800.049.3729554
AGCAGGG163500.048.5785458
CGAAAGC56450.045.212044
TCGTACT9100.044.5889132
AAGCAGG342600.043.266427
GCAGGTA84950.043.1809359
AAAGCAG360350.041.2308436
GTTACGT1905.456968E-1239.8098451
AAAAGCA322350.038.326855
GCACGCC4250.036.880558145
GGATAGC16550.036.5770875
ACTCCGA2600.034.381231
TTACGGG5250.032.569843145
ACAAGGG47000.031.4718383
GGTCGTT8700.030.834467
Produced by FastQC (version 0.11.5)