FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n02_TR_PLASMID_CONTROL_P1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n02_TR_PLASMID_CONTROL_P1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2508815
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT98050.3908219617628243No Hit
CTTCTAATAGATGGCACAGCATCATTGAGCCCTGGGATGATGATGGGCAT72860.29041599320794875No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG72040.28714751785205367No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG57200.22799608580146405No Hit
GATGTATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGT55540.2213794161785544No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT54610.21767248681150267No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT54310.21647670314471176No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA51740.20623282306586974No Hit
GTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA49230.19622809972038593No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT47610.1897708679197151No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA43120.17187397237341137No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG42150.1680076051841208No Hit
TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC38620.15393721737154792No Hit
ATCTGGGACAGGCCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTG36500.14548701279289228No Hit
ATTGTATGTTGGTTCTCCAGGGCAACAAGAAGCTCCGCGTTGTATGACCA35960.14333460219266864No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC34540.13767455950319174No Hit
ATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG34180.1362396191030427No Hit
ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT33580.13384805176946088No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGGTACA32830.13085859260248364No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT32690.1303005602246479No Hit
AACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAG32110.12798871180218552No Hit
GTCCTGGGGTTGACCAGAGGGCCCCGGGCGCTCCGTGTGTGGCTGCGATG31190.12432164189069342No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTTT30950.12336501495726068No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG30230.12049513415696256No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT29180.11630989132319443No Hit
ACATATAGGTGCCATAGAGGAGACACACAAATTCAGACGAGAAGATCATT28730.11451621582300807No Hit
CTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAAGAAATGGGACCT28130.11212464848942627No Hit
CTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGC28090.1119652106671875No Hit
ATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGC27830.11092886482263538No Hit
TCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTT27590.10997223788920267No Hit
CCTATATGTATATCTGTAGTCTTTGATGAACAATTGGAGAGCCATCTGGG27270.10869673531129237No Hit
ATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT27160.1082582813001357No Hit
TCTCTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCA27150.10821842184457603No Hit
TCTAATAACAGTTTCTTTAGTAGATTGAATTGGTTGACCCACTTAAAATT26990.10758067055562089No Hit
GAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTT26090.10399331955524821No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT26050.10383388173300939No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG25950.10343528717741243No Hit
ATGTATATCTGTAGTCTTTGATGAACAATTGGAGAGCCATCTGGGCTGTT25890.10319613044405428No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT25620.10211992514394246No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT25390.10120315766606945No Hit
GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT25110.10008709291039794No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCCC3300.084.04339145
CCGTTCC2650.056.354206145
TCGAGCC1051.3169483E-954.18182145
ACATCTT3450.053.592888145
CGTAGGG400.006532232753.335228145
TACTGAT12000.050.6921587
CATATAC11800.050.374293
GTCGGCC1152.9504008E-949.470356145
ACTGATG12300.048.4521188
ACCGGCC2850.047.409092145
GTTCGGG605.440527E-447.409092145
CTTCTAA20950.047.0659831
CGACGAA7200.045.433712145
AATATTT2350.045.391685145
TTTAGTT1101.1601696E-745.254135145
TCTGACC2050.045.096455145
CAAGGCC2050.045.096455145
TTCGGAC2050.044.3467183
TAGATGG22050.043.8802838
TTCTAAT22450.043.6097562
Produced by FastQC (version 0.11.5)