FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n02_TR_DEMUXING_CONTROL_P1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n02_TR_DEMUXING_CONTROL_P1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG80.4750593824228029No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG50.29691211401425177No Hit
ATCACTCACTGAGTGACATCAAAATCATGGCGTCCCAAGGCACCAAACGG50.29691211401425177No Hit
GTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGAGTG40.23752969121140144No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA40.23752969121140144No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA40.23752969121140144No Hit
TCTCTGTAGTGACTTCAAATTATAACAGGAGATTTACCCCGGAAATAGCA30.17814726840855108No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACA30.17814726840855108No Hit
TCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCCA30.17814726840855108No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC30.17814726840855108No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA30.17814726840855108No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT30.17814726840855108No Hit
CATTTTAAGTGCCTCATCGGATTCTTCTTTCAGAATCCGCTCCACTATCT30.17814726840855108No Hit
CTTTCAGTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAA30.17814726840855108No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG30.17814726840855108No Hit
AAACTATTCTCTGTTATCTTCAGTTTGTGTCTCACTTCTTCAATCAACCA30.17814726840855108No Hit
CCGCTATATGATGCAATCAAATGCATGAGAACATTCTTTGGATGGAAGGA30.17814726840855108No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT30.17814726840855108No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG20.11876484560570072No Hit
GGTTCTCATCGCTTGCACCATTTGTCTAGCCTGACTAGCAACCTCCATGG20.11876484560570072No Hit
GAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGT20.11876484560570072No Hit
GTCTTCATGGTGGCAAAATGGCAGTGAGCTGCTCCAGGTTTGTCTTCATG20.11876484560570072No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG20.11876484560570072No Hit
TTACTGGACCTTGCTAAAACCCGGAGACACAATAATATTTGAGGCAAATG20.11876484560570072No Hit
ACCTAACTGACATGACTCTTGAGGAAATGTCAAGGGACTGGTCCATGCTC20.11876484560570072No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG20.11876484560570072No Hit
CCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAAATACGTCGGAGAGTT20.11876484560570072No Hit
GTGTATCCTGTTCCTGTCCCATGGCTGTAAGGAGGGTCTCCAGTATAAGG20.11876484560570072No Hit
GCAGATGTATCCTATTTGATAATCCAGGTTTTGATCGAAAGACACCCATG20.11876484560570072No Hit
GATATGTTCCTTCTATTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCC20.11876484560570072No Hit
GTAATATTTAAAACTGCCAGGGTCATCAAAACCAAGGACAGTTTGAAAAA20.11876484560570072No Hit
GCAGGTCGGTCTGCTGGGCCAGCGCCATGGCGATCCACACCCGCTGGCGC20.11876484560570072No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC20.11876484560570072No Hit
TACCAGGCCAAATGCCACTTCAGTGGTCACAGCCCCCATCCTGTTGTATA20.11876484560570072No Hit
GTTGTATGGGCCTCATATACAACAGGATGGGGGCTGTGACCACTGAAGTG20.11876484560570072No Hit
ACTGAAGGATGAGCTAATTCTCAGTCCCATTGCAGCCTTGCATATATCCA20.11876484560570072No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA20.11876484560570072No Hit
GTGGTAGAACTCAAAACATCCATTTCCGATTTCTTTGGCATTATTCTTTA20.11876484560570072No Hit
CAATAACTTACTCATCGTCAATGATGTGGGAGATTAATGGTCCTGAATCA20.11876484560570072No Hit
GGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGA20.11876484560570072No Hit
TAACCATACCATCCATCTATCATTCCAGTCCATCCCCCTTCAATAAAACC20.11876484560570072No Hit
GGATACATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCCGAAGATGG20.11876484560570072No Hit
GTATGGTTATCATCATCAGAATGAACAGGGATCAGGCTATGCAGCGGATC20.11876484560570072No Hit
ACTTGAACCGTTGCATCTGCACCCCCATTCGTTTCTGATAGGCCTGCAAA20.11876484560570072No Hit
GTTGACACAGTACTCGAGAAGAATGTGACAGTGACACACTCTGTTAACCT20.11876484560570072No Hit
GGCTAAAACCATTCTGTTCTCATGTCTGATTAGTGGATTGGTTGTTGTCA20.11876484560570072No Hit
GACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTGGCCAAGACCA20.11876484560570072No Hit
GTTCACAGGTTGCACATACCAGGCCAAATGCCACTTCAGTGGTCACAGCC20.11876484560570072No Hit
AAGCCGAGATCGCACAGAGACTTGAAGATGTCTTTGCAGGGAAGAACACT20.11876484560570072No Hit
CTTCTATTGTGTTGGCCAATGCTGTTGCAGCAGGTTGGTTTCTATTTAGA20.11876484560570072No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG20.11876484560570072No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC20.11876484560570072No Hit
GCTAGGATGAGTCCCAATGGTTCTCATCGCTTGCACCATTTGTCTAGCCT20.11876484560570072No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT20.11876484560570072No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA20.11876484560570072No Hit
TTCATAAGCAATTCTTGTTTTTCGTCCATTCTCACCCCTCCAGAAGTTCC20.11876484560570072No Hit
GTGTATGTTGATGGAGCAAACGGAGTAAAGGGATTTTCATATAGGTATGG20.11876484560570072No Hit
GGTCTGAAAAATGATCTTCTTGAAAATTTGCAGGCCTATCAGAAACGAAT20.11876484560570072No Hit
AGATAACATTCCATGGGGCCAAAGAAATCTCACTCAGTTATTCTGCTGGT20.11876484560570072No Hit
ATTATTAGCTTGGCGCCAGATTCGCCTTATTTCTTCTTTGTCATAAAGGA20.11876484560570072No Hit
AATCAGACATGAGAACAGAATGGTTTTAGCCAGCACTACAGCTAAGGCTA20.11876484560570072No Hit
GCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATCTTCAGTTTGTGT20.11876484560570072No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT20.11876484560570072No Hit
GCAGGGAAGAACACTGATCTTGAGGTTCTCATGGAATGGCTAAAGACAAG20.11876484560570072No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA20.11876484560570072No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT20.11876484560570072No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT20.11876484560570072No Hit
TCACTATTCACGCCACAAAAAGAAATGCTGCTCGCACTAGTCCAGATTGT20.11876484560570072No Hit
GGATACGCCGTTCATCAAGTGTTTGTAAAAGTTGGGCATAGAATCTTTTT20.11876484560570072No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG20.11876484560570072No Hit
GAGTAGAGGCTTTGGGTCCGGCATCATCACCTCAAACGCATCAATGCATG20.11876484560570072No Hit
ATCCTGTCACCTCTGACTAAGGGGATTTTAGGATTTGTGTTCACGCTCAC20.11876484560570072No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA20.11876484560570072No Hit
GAGCGAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT20.11876484560570072No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC20.11876484560570072No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACC20.11876484560570072No Hit
CTTCTCACTATGTTCCTAGCAGCAATAATCAAGCTTTGATCAACATCATC20.11876484560570072No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC20.11876484560570072No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT20.11876484560570072No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA20.11876484560570072No Hit
CTGCAGATGTATCCTATTTGATAATCCAGGTTTTGATCGAAAGACACCCA20.11876484560570072No Hit
AATTCTCACTATGAGGAATGTTCCTGTTACCCTGATACCGGCAAAGTGAT20.11876484560570072No Hit
TAGCTGTAGTGCTGGCTAAAACCATTCTGTTCTCATGTCTGATTAGTGGA20.11876484560570072No Hit
CCCATGGCCGGTTCGAACCATGCCAGTTGTCTCTGCACACACACATCACT20.11876484560570072No Hit
CCGGAATGGATCCCAGGATGTGCTCTCTGATGCAAGGTTCAACTCTCCCT20.11876484560570072No Hit
GTCAAGTGCAAGATCCCAATGATATTTGCGGCAATAGCGAGAGGATCACT20.11876484560570072No Hit
GTCCTGGAAGAGAAGGCAATGGTGAAATTTCGCCAACAATTGCTCCCTCT20.11876484560570072No Hit
GTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGACATGGGTTTGAG20.11876484560570072No Hit
GGACCACAGCTGCCTGTTCCATCTTCGGGACGCGGGTTGTCACCGAAAAC20.11876484560570072No Hit
GGCTAAAGACAAGACCAATCCTGTCACCTCTGACTAAGGGGATTTTAGGA20.11876484560570072No Hit
CTATGAGACCGATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCACATAC20.11876484560570072No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA20.11876484560570072No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT20.11876484560570072No Hit
TTCTTTCATAAGCAATTCTTGTTTTTCGTCCATTCTCACCCCTCCAGAAG20.11876484560570072No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG20.11876484560570072No Hit
CATTAAGGCCCTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCT20.11876484560570072No Hit
ATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA20.11876484560570072No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT20.11876484560570072No Hit
CAATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCA20.11876484560570072No Hit
TTCCAGCACGTGTGGCTGTCTCGATGTCCAGACCGAGGGTGCTGCCCCTT20.11876484560570072No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG20.11876484560570072No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGCAT100.008060887134.56254
TAATGGT100.008060887134.56255
Produced by FastQC (version 0.11.5)