FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n02_TR_CW_E2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n02_TR_CW_E2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1554292
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA151550.9750420126977428No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG97330.626201511685063No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA84970.5466797744567944No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG56250.3619011099587465No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG54070.34787543138612304No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA49690.3196953982906687No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT42360.2725356625396No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG40420.2600540953694672No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA40190.25857432194208035No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT35230.2266626862906069No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA31760.20433740892959623No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA30770.1979679493943223No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT29470.18960401263083126No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT28550.18368491892128377No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA28270.18188345561837801No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT27420.17641472773455694No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG26040.16753608717023571No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT25600.16470521626566953No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT25590.16464087829056576No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC25550.16438352639015064No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC25040.161102289659858No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC24390.15692032127811248No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT22840.14694793513702703No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG22310.14353802245652683No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT22250.14315199460590416No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT22090.14212258700424374No Hit
GAATGAGGAATCCCCTCAGAACAGCGGACTCCACTCCAGCTGTGCCTTCA21850.1405784756017531No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT21760.1399994338258191No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT20890.1344020299917905No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA20330.13079910338597897No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA20110.12938366793369585No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA19670.12655279702912967No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT19160.12327156029883703No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGA19150.12320722232373325No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA18710.12037635141916707No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG18110.11651607291294043No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC18100.11645173493783664No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACA18010.11587269316190266No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC17700.11387821593368556No Hit
GTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGAATGAGGAATCCCCTC17540.11284880833202512No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGT17490.11252711845650624No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC17330.1114977108548458No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT17000.10937455767642117No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT16890.10866683995027962No Hit
GTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGAGTG16690.10738008044820406No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAAGGC16480.10602898297102474No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT16310.10493523739426053No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA16100.10358413991708121No Hit
GAGTAGAGGCTTTGGGTCCGGCATCATCACCTCAAACGCATCAATGCATG16070.10339112599176989No Hit
GCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGAATGAGGAATC16060.10332678801666612No Hit
CTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTG15980.10281208421583589No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAAAC15750.10133231078844902No Hit
TCCTCATTCTGGGCAAAGAAGACAAGAGATATGGGCCAGCACTAAGCATC15650.10068893103741125No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTT100.0075110127142.23721145
TCTAAAC100.0078810565139.967353
GAGCGAA11900.0135.84631
ACCTCCC352.1755113E-9121.9176145
TATTAGT352.4338078E-9119.968151
TTAGGGG257.1148515E-6113.78977145
AATGTTT257.1148515E-6113.78977145
ATTAGTA1900.0103.130511
GCAAAAG70000.0101.076423
AGCAAAA71750.098.900572
AGCAGGC5300.094.005778
ATCCGCC406.861392E-788.89826145
GCAGGGG13050.088.7592549
GCAGGGT31400.086.968139
CGGTGCC250.001013318885.34233145
CGCCGGG250.001013318885.34233145
TCACGGA250.001080316783.9777152
GAGCAAA86650.082.459351
AGCAGGG48700.080.1157468
TTAGTAG2450.079.978772
Produced by FastQC (version 0.11.5)