FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n02_TR_CW_B6_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n02_TR_CW_B6_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4242225
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT530861.2513716269174784No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG293020.6907224392859879No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC291540.6872337040114562No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA274610.6473254011750909No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA273470.6446381321122759No Hit
GTAGTAAACAGTATTTGCAACACTACAGGGGCTGAGAAACCAAAGTTTCT193760.4567414505359805No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT192180.45301698990506156No Hit
GTAGTAAACATGACCAAAGAGTTCTTTGAGAACAAATCAGAAACATGGCC187130.4411128594075043No Hit
GTTTACTACTGTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCAA167020.3937084902380237No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA157800.37197461237911705No Hit
TGTTTACTACTGTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCA146690.345785525284491No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA142950.336969396955607No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG132480.31228895214185953No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT113250.2669589661085869No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA101970.2403691459081025No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT99410.2343345767845883No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC98660.23256663661168375No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT96350.2271213808791377No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT86750.20449174666595948No Hit
CCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAG81310.1916682872784918No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA74520.17566253557979597No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA74280.17509679472446651No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT73240.17264525101803888No Hit
GGAACACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGG71200.1678364537477385No Hit
GGAACAGGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGA70880.16708213260729923No Hit
GTATATGTGAACTTCTCTCCTTGTTACTCCAATTTCGATGAATCTATTCT70310.16573849807589178No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA68880.16236762547955377No Hit
GTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCAAATCTGTGCTT68630.1617783120885856No Hit
GTGCTGAGTCCTCTGTCAAAGAGAAAGACATGACCAAAGAGTTCTTTGAG65500.15440010843366395No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT65400.15416438307727667No Hit
GGTAGAAACTTTGGTTTCTCAGCCCCTGTAGTGTTGCAAATACTGTTTAC64610.15230215276181722No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG64590.15225500769053976No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT63630.14999204426922194No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT62660.1477055083122654No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC61860.1458197054611672No Hit
GATTTGTATGATTACAAGGAGAATAGATTCATCGAAATTGGAGTAACAAG61620.14525396460583775No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA59770.1408930455126732No Hit
AGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT59310.13980870887329172No Hit
GTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGAT58610.1381586313785808No Hit
GTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGATTCAGCTGAAA58520.13794647855783226No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG56900.13412772778435844No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC55000.12964894601300025No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT54020.12733883752040498No Hit
AGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGT53800.12682024173635298No Hit
CCTTTGGGGGACTCTCCAATGGGCCATGTTTCTGATTTGTTCTCAAAGAA51830.1221764522155237No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA51750.12198787193041388No Hit
TCCTCAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAG51560.12153999375327806No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA50140.11819269369257879No Hit
GCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCA50050.11798054087183024No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC49270.11614188309200951No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT49110.11576472252178988No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA48920.11531684434465406No Hit
AGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC48700.11479824856060203No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC48460.11423250770527257No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT47600.11220526964034203No Hit
CACATATACTATCTGGAAAAGGCCAATAAAATTAAATCTGAGAAAACACA46110.10869296183017166No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA45870.1081272209748422No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAAAC45450.10713717447801566No Hit
ACAAACACCGAAACTGGAGCACCGCAACTCAACCCGATTGATGGGCCACT45090.10628856319502147No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT44770.1055342420545822No Hit
ATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAG44530.10496850119925275No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG44330.1044970504864782No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA43870.10341271384709674No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG43760.10315341595507074No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA43740.10310627088379329No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG43310.10209265185132801No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA34350.0135.022061
AGCAGGC30850.0105.666228
GCAAAAG248150.0104.857883
GAGCAAA259950.0100.852681
GCAGGGG40450.093.653359
CAAAAGC287000.090.7463764
AAGCAGG342100.089.6136257
CGATATA406.8761256E-788.87081145
GCAGGGT85800.085.437819
AGCAGGG131050.085.184438
TATTAGT1850.083.315231
AAAAGCA332000.078.444565
AGCGAAA59250.078.396852
AGGTTCC13050.076.81707145
ACGGCCC756.730261E-1175.836426145
GTCGCCC3700.074.93971145
CCTGTTT3050.074.59321145
AGCAAAA359700.072.767912
AAAGCAG425750.072.157246
TGACGCC6950.071.60813145
Produced by FastQC (version 0.11.5)