FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n02_TR_795-7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n02_TR_795-7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477105
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG53040.3590807694781346No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA35150.23796547977293422No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA34780.2354605799858507No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT28910.1957206833637419No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC21480.14541958763933507No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA20390.1380402882665755No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG20320.137566388306857No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC18300.1238909894692659No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT17700.11982898981453587No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC17440.11806878996415285No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT16450.11136649053384831No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA15760.10669519093090876No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA15640.10588279099996276No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA15430.10446109112080726No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC15220.10303939124165175No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT14810.10026369147758621No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5200.0125.1738741
ACATCGT250.001080234983.97916
GAGCAAA51600.074.872691
TCGTACT300.002226329969.989692
TGACGCC4350.067.03403145
GCAGGTA13900.066.1900949
AGCGAAA10900.065.4949341
AAGCAGG72500.061.8742527
CGAAAGC12100.058.9995733
GCGAAAG12250.058.277132
AGCAGGT27500.053.3456888
TCGCGCC553.539502E-451.72471145
AAAGCAG87200.051.2831086
CAAAAGC75900.050.6215254
AGCAAAA78850.048.5534062
GCAAAAG79750.047.8299453
GTAGAAA12950.047.5605661
GACAGTA4600.047.1621747
AGTCGGC1059.122414E-846.6566355
CACCTAC3750.044.7934043
Produced by FastQC (version 0.11.5)