FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n02_TR_740-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n02_TR_740-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022270
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG13030.12746143386776487No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC12570.12296164418402183No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC10940.10701673726119323No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA3050.057.365641
GTTACGT2900.053.092891
TTACGTG2950.052.193012
TACGTGT3000.051.3231243
AGCGAAA5200.049.797791
CGAAAGC5700.049.113043
GCGAAAG5500.048.354022
ACTCCGA2200.047.717781
GAGCAAA29500.047.448191
TGACGCC2250.044.248356145
ACGTGTT3750.042.9226954
GCCGACC2500.042.668056145
CAAAAGC34850.039.7605934
CGTGTTG4350.037.0023235
GATATAC3450.036.5144773
CGACTCC2700.036.28026
CGAAGCG2550.035.6774335
AGACGAC2750.035.6292763
GCAAAAG39100.035.6195143
TCTCGGG4400.035.556713145
Produced by FastQC (version 0.11.5)