FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n02_TR_644-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n02_TR_644-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977665
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA28500.067.285231
CGAATAA2850.064.91331145
GACGAAA4750.064.41398145
GAGCGAA6350.062.8225021
GCGAAAG10150.055.1617472
CAAAAGC34900.054.5369534
AGCGAAA10200.054.2052081
GCAAAAG35200.053.4839823
AGCAAAA36800.050.9684072
CGAAAGC11250.050.390263
AAAGCAG54550.042.203516
AAAAGCA47200.040.6215065
GCCGACC2001.0742951E-832.019737145
CGAAGCG1153.8226566E-430.5178939
GTCGCCC2152.1720552E-829.785803145
AAGCAGG75850.029.2449657
AGACGAC1556.994326E-527.0915363
ACGACTC2001.4022469E-524.4915055
TGACGCC3052.4816472E-823.3295145
GACGACT1801.933123E-423.3252434
Produced by FastQC (version 0.11.5)