FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n02_TR_562-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n02_TR_562-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1554143
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG33240.2138799325416001No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA23510.15127308104852644No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC18880.12148174267104123No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC16090.10352972667251341No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT15820.10179243480168813No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4100.0104.010861
TCGCGCC301.7553673E-594.87593145
TGACGCC3650.066.28319145
GTTACGT1052.3646862E-1159.921951
AGCGAAA7400.057.6276472
TACGTGT1103.6379788E-1157.1982273
TCCGAGG2700.056.9628453
GCAGGTA8050.053.1859
ACTCCGA2250.052.820091
GAGCAAA45800.051.439551
GCGAAAG8300.051.3788643
CGAAAGC8450.049.636294
AAGCAGG63750.043.635287
CGAAGCG2100.043.2713975
CTCCGAG3450.042.5532722
GACGAAA10250.041.652847145
GCAAAAG55850.041.557333
AGCAAAA55700.041.543732
CGAGGTG3900.041.223055
GTAGAAA10750.040.969891
Produced by FastQC (version 0.11.5)