FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n02_TR_559-3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n02_TR_559-3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1289997
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGG350.003841885660.987835145
CGTCGAG350.00410257159.9852452
TCGTCGA350.00410320159.9829181
CGAATAA4600.057.231335145
TGACGCC3250.054.732677145
GAGCAAA32950.054.5823331
GCGAAAG10300.052.9966772
CGAAAGC10750.050.7782063
GACGAAA6500.050.35406145
AGCGAAA11450.047.6720161
GAGCGAA9800.044.987191
GCAAAAG40600.044.1270753
CAAAAGC41750.042.909944
AGCAAAA43100.040.7556342
GCCGACC2400.038.54092145
AAAGCAG59550.036.545696
CGAAGCG1551.8853189E-631.7022299
GTCGCCC2705.456968E-1231.623322145
GTCGTTG900.00431466231.1022571
AAAAGCA62000.028.3306435
Produced by FastQC (version 0.11.5)