FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n02_TR_545-3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n02_TR_545-3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954581
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA8150.084.104071
GCGAAAG8300.081.741432
CGAAAGC9500.071.416193
GAGCAAA20750.056.966191
GAGCGAA3350.054.284661
TATACGA553.846857E-450.8574877
CAAAAGC24850.046.434194
GCAAAAG25650.044.9930233
AGCAAAA27550.041.8900532
GACGAAA3600.033.58866145
AACGCAA950.005189755529.949022145
TCGCCGG3350.029.725523145
AAAAGCA39700.029.5936875
AAAGCAG50600.027.0871376
CTATACG1307.9958345E-426.9014233
GAAAGCA28150.025.3397354
ATATACG1500.001843648823.309686
TGACGCC2553.194773E-622.314959145
GCCGTAA2553.194773E-622.314959145
GTCGCCC1650.002905506621.55422145
Produced by FastQC (version 0.11.5)