FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n02_TR_540-7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n02_TR_540-7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441556
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC29760.20644359289545464No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC24840.17231380536031898No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA24020.1666255074377964No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG23570.16350388052909495No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA22380.15524891159275117No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA21810.15129485084172936No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG18350.12729300838815835No Hit
CCATCTCAACAGATGCAGAAAAAGCATTTGATAGCGCTGCTACCAAATCA16690.11577767356939309No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC16080.11154613487093112No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG15490.10745333514618925No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA15040.10433170823748782No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG14680.1018344067105267No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT14600.10127945081564642No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4950.0100.4746861
TTACGTG2650.084.5877762
GTTACGT2950.075.985631
AGCGAAA7000.071.049952
TACGTGT3200.070.0492553
CGAAAGC7150.069.5521554
GCGAAAG8200.062.360923
GCGATTA2000.056.898174145
GTCACGT2850.056.5309751
TCTCGGG3150.054.18874145
ACTCCGA3250.053.8840451
AGTCGGC1600.052.529655
GAGCAAA41750.052.18041
ACGTGTT4550.049.260284
AATGGGT9900.047.395567
TCGCCGG3200.044.451702145
TATGTGT9900.043.869233
CTCCGAG4350.043.4788482
ATGGGTG11000.042.7598348
GTTATGT10550.042.4943351
Produced by FastQC (version 0.11.5)