FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n02_TR_528-3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n02_TR_528-3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194139
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7050.086.299891
GAGCAAA35100.063.3578031
AGCGAAA9650.061.5987242
GCGAAAG10150.057.8753173
CAAAAGC41500.053.07924
CGAAAGC11200.052.4473084
GCAAAAG42550.051.771543
TGACGCC2500.051.202938145
AGCAAAA46600.046.9719432
AAAGCAG63400.043.8954356
GCCGACC2450.040.637253145
AAGCAGG75150.035.5434767
GTTACGT1804.5529305E-934.9663351
GTCGCCC2053.583409E-1034.69034145
AAAAGCA66350.033.3048975
GTAACGG1551.9416675E-631.5824971
GCGATTA950.005195183729.94324145
CGCCGTT2202.7319402E-829.092577145
CGAAGCG1703.875881E-628.9023349
CGTGTAA5050.026.75621145
Produced by FastQC (version 0.11.5)