FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n02_TR_427-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n02_TR_427-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314807
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT24690.18778421471744522No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG23150.17607146904450616No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT22590.17181228879980104No Hit
GATTCGCTTGGAGAAACTGTGATGAGAATGGGAGACCTTCACTACCTCCA21440.16306575794013875No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC20470.1556882493019888No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA19590.14899525177459505No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT19520.14846285424400693No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA18090.13758673326199206No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC17400.1323388147461947No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT16070.12222326166502002No Hit
GAAAGATGACCTTCTTGAAAATTTGCAGGCCTACCAGAAGCGAATGGGAG15480.11773591105006286No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG14560.11073868636233303No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG14460.10997811846149283No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT14050.10685979006804802No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT13470.10244849624317484No Hit
CTTGAATGGAATGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATT13440.10222032587292279No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2650.089.743551
AGCGAAA9100.085.320191
CGAAAGC10450.074.297963
GCGAAAG10650.072.9026952
CCGACGC1405.456968E-1250.810764145
CAATTCG2950.035.7117169
TGACGCC3450.035.052063145
GTCATAC4800.034.9735951
CTGCACG850.003268959532.916321
TCTACAC5850.032.2833143
ATCCCGA3600.031.0829076
GAAAGCA27700.028.2808174
GTCCGGA1000.00723735827.9788722
CTACACT7150.027.3908624
GTAGAAA16100.026.936181
GGTTCGG1050.00918906426.6424946
CCTTACC7200.025.68766145
GATAGAC5450.025.6686922
CTGCCGC3359.458745E-1125.481218145
GACGCTC1400.001116825725.405382145
Produced by FastQC (version 0.11.5)