FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n02_TR_286-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n02_TR_286-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1037047
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA15650.15090926447885197No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC14160.13654154536872487No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG14080.13577012420844956No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC14000.1349987030481743No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC13840.13345586072762372No Hit
CCATCTCAACAGATGCAGAAAAAGCATTTGATAGCGCTGCTACCAAATCA11980.11552031875122343No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA10400.10028475083578661No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG10380.10009189554571779No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA3900.078.9287641
GCCGACC2350.063.587444145
AGCGAAA5300.056.7596632
GTTACGT2000.052.469681
TTACGTG2000.052.469682
CGAAAGC5850.051.423284
GAGCAAA31400.051.2442781
GCGAAAG5900.050.9874883
TACGTGT2100.049.9711273
TGACGCC2950.048.24229145
AATGGGT6300.044.4123577
CAAAAGC36500.043.317444
ACTCCGA3000.041.975751
GGTGCGA2200.041.4557238
AAAGCAG48500.038.9409376
CGAAGCG2350.038.7010465
GTTATGT7450.037.5621951
AAGCAGG50200.037.2041977
GCAAAAG42300.037.0471573
TACTGCG1702.593879E-937.0374265
Produced by FastQC (version 0.11.5)