FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n02_TR_283-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n02_TR_283-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208316
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG32270.2670658999798066No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA21580.17859566537230329No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC15960.13208465335226877No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG15480.12811218257475693No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC14760.12215347640848917No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA14660.12132587832984086No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC13480.11156022100179092No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA13130.10866362772652188No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4250.0107.107541
CGATTAA502.595516E-885.330956145
GTTACGT2400.072.9498441
TGACGCC2700.071.10913145
AGCGAAA6700.067.9413452
GCAGGTA8100.062.4729239
GCGAAAG7700.061.8488733
CGAAAGC7600.059.8931854
GGTTATA1550.058.736391
TACGCAG502.3862059E-456.0231635
GTCGTCT651.2267352E-554.69933145
GAGCAAA39650.054.4005281
AAGCAGG54950.048.045347
GCGACTA1501.4551915E-1146.6859664
GTTATGT5750.045.0639721
AGCAGGT15050.044.3364528
CTATAAC804.583746E-543.7680934
CGAAAGT658.70341E-443.094744
CAAAAGC51000.042.4293064
AAAGCAG62150.042.1413156