FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n02_TR_199-7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n02_TR_199-7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920770
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA13780.1496573519988705No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC11560.12554709645188267No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA11450.12435244415000489No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA10890.11827057788589984No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG10880.11816197313118369No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC10660.11577266852742812No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2450.0114.380511
AGCGAAA4050.069.193152
CGAAAGC4150.065.830554
GCGAAAG4700.062.6050833
TGACGCC2200.058.15096145
CGGAGAT851.7120328E-857.688615
GGTTATA1650.055.1972661
GCCGACC1700.054.350246145
GTCGCCC1750.052.797382145
GGTCGTT1153.3687684E-948.728117
AGTCGGC1059.050564E-846.7003065
GATAGCT1950.046.6977737
AGCAGGT5900.046.460998
GAGCAAA22650.045.4681471
CCCGCTC3500.044.03653
AGCTACT2100.043.511098
TTACGTG1455.3842086E-1043.4843182
TATGTGT5350.043.2133873
AAGCAGG30600.043.0351947
GTTACGT1507.512426E-1042.034841
Produced by FastQC (version 0.11.5)