FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n02_TR_197-7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n02_TR_197-7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1273345
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG24580.19303488057046597No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA17870.14033902830733225No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG14590.1145801020147721No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC14110.11081050304512917No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA13330.10468490471945938No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA3050.0116.9463961
GTTACGT1600.078.680851
TTACGTG1750.071.93962
TACGTGT1800.069.9412843
GGTTATA1650.067.819181
AGCGAAA5650.063.1328352
AGTCGGC1500.060.6110155
ACGGTAT350.00411348759.944966
CGATTAA607.644208E-659.245453145
CGAAAGC6050.058.9564554
GATATAC2800.057.451773
GCGAAAG6600.055.105253
GAGCAAA34550.053.440731
GCAGGTA6800.050.5756659
ACTCCGA2500.047.55821
AAGCAGG50600.044.5028427
TCCGAGG2700.044.0371063
TATGCGT1751.8189894E-1243.9596336
TGACGCC2800.043.164547145
CCGCCCG658.7591226E-443.0390971
Produced by FastQC (version 0.11.5)