FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n02_TR_156-7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n02_TR_156-7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1546677
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG25160.16267132698035855No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC23910.1545894844237032No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC23510.1520032948055735No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG20370.1317017063032553No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA19800.12801638609742047No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA18950.12252073315889485No Hit
CCATCTCAACAGATGCAGAAAAAGCATTTGATAGCGCTGCTACCAAATCA18870.12200349523526889No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT18170.11747766340354192No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA17540.11340441475498762No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC16050.10377085842745448No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA3300.082.643491
TGACGCC3700.069.20266145
GTCGTCT1200.065.197876145
GCCGACC3200.062.234333145
GTTACGT3050.061.9044571
AGTGCGA400.00698486452.4383546
ACGGTAT400.00698486452.4383546
CGAAAGC5500.052.123924
AGCGAAA5850.046.6194042
AAACGCC952.1923152E-644.921024145
GATAGCC2800.043.18301145
GCGAAAG7250.042.4397353
TGAAGCG3150.042.175375
GCTACTA4000.042.1154259
GTAATGC2400.040.791981
ACTCCGA4800.040.791981
TCGCCGC2150.039.697647145
TCTCGGG5750.039.58258145
GAGCAAA41100.038.4525071
GATATAC5300.038.2630963