FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n02_TR_123-7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n02_TR_123-7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2282898
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC33670.14748797361949592No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA30150.13206897548642121No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA29580.12957214908419035No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG29020.12711912665392847No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC27570.12076755071842894No Hit
CCATCTCAACAGATGCAGAAAAAGCATTTGATAGCGCTGCTACCAAATCA26360.1154672701101845No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC26130.11445977875489838No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA23820.10434106123006809No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5650.084.29531
GTTACGT3450.073.084681
TTACGTG3850.065.49292
TACGTGT4150.060.758473
TGACGCC6700.059.427147145
AGCGAAA10400.057.917261
CGAAAGC10800.056.4219323
GCGAAAG11900.051.7950362
ACTCCGA6950.047.364831
TCTCGGG7450.045.80961145
GCCGACC5450.043.05158145
TCCGAGG7700.042.7523083
GAGCAAA61700.042.5685651
GTTATGT14300.040.16251
AGTCGGC2500.039.2160955
GTATATC7300.038.37781
CTCGCAA1651.9172148E-938.204193
CTCCGAG9150.036.742832
TATGTGT15500.036.6020773
AATGGGT14300.036.2362677
Produced by FastQC (version 0.11.5)