FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11206548
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG118820.10602729761207466No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG113010.10084282867480691No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA22000.0114.876661
GAGCAAA200300.073.217081
GCAAAAG204800.071.2209553
GCGTAGT300.002085970771.151476
AGCAAAA217750.067.052752
GCAGGGT114350.055.1913879
AGCAGGG191100.047.583248
CGAAAGC59100.047.5547033
GCAGGTA97550.047.337069
TCGTACT8150.047.1447752
GGATAGC17050.046.7387965
CAAAAGC348950.042.2484134
AAAGCAG416050.041.608346
GCACGCC907.2830175E-540.467285145
AAGCAGG450250.038.416267
CTATTCG11800.036.178719
GGTCGTT9450.034.6345757
AAAAGCA429550.034.536335
GTTATGT5850.032.8574641
GTCATAC16700.031.9720821
Produced by FastQC (version 0.11.5)