FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n01_TR_PLASMID_CONTROL_P1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n01_TR_PLASMID_CONTROL_P1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2508815
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT100290.39975047980819634No Hit
CTTCTAATAGATGGCACAGCATCATTGAGCCCTGGGATGATGATGGGCAT81840.32620978430055625No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG73770.29404320366388115No Hit
GATGTATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGT61790.24629157590336476No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG60510.24118956559172358No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA55800.22241576202310653No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT55050.21942630285612927No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT54540.21739347062258474No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA49400.19690571046490074No Hit
GTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA46350.18474857651919332No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG43610.1738270856958365No Hit
TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC40780.16254685977244238No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC38810.15469454702718216No Hit
ATCTGGGACAGGCCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTG36450.14528771551509378No Hit
AACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAG35820.1427765698148329No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGGTACA35470.14138148887024352No Hit
ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT35150.14010598629233323No Hit
ATTGTATGTTGGTTCTCCAGGGCAACAAGAAGCTCCGCGTTGTATGACCA34630.13803329460322902No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTTT33650.13412706795837875No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT33350.13293128429158785No Hit
GTCCTGGGGTTGACCAGAGGGCCCCGGGCGCTCCGTGTGTGGCTGCGATG31530.12567686337972309No Hit
ACATATAGGTGCCATAGAGGAGACACACAAATTCAGACGAGAAGATCATT30960.12340487441282039No Hit
TCTAATAACAGTTTCTTTAGTAGATTGAATTGGTTGACCCACTTAAAATT30570.12185035564599223No Hit
CTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGC30050.119777663956888No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG29990.11953850722352982No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT29760.11862173974565682No Hit
CTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAAGAAATGGGACCT29760.11862173974565682No Hit
CCTATATGTATATCTGTAGTCTTTGATGAACAATTGGAGAGCCATCTGGG29410.11722665880106743No Hit
ATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT29280.11670848587879139No Hit
CCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGG29270.1166686264232317No Hit
ATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGC28930.11531340493420199No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT28810.11483509146748565No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT28770.11467565364524687No Hit
TCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTT28740.11455607527856776No Hit
CTAAAATAGATCTCTGGTCATACAACGCGGAGCTTCTTGTTGCCCTGGAG28210.11244352413390386No Hit
GAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTT26850.10702263817778514No Hit
TTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCC26840.10698277872222543No Hit
TGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATC26780.10674362198886725No Hit
ATGTATATCTGTAGTCTTTGATGAACAATTGGAGAGCCATCTGGGCTGTT26750.10662404362218818No Hit
GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT26680.10634502743327029No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG26330.10494994648868092No Hit
GTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAG26310.10487022757756151No Hit
ATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTT26070.1039136006441288No Hit
GTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCAACAGGGATGCGA25910.10327584935517366No Hit
GATATACATATAGGTGCCATAGAGGAGACACACAAATTCAGACGAGAAGA25650.10223950351062154No Hit
GCATTAATGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGC25630.10215978459950216No Hit
TTCACTATGAGGGCAAAATCGTCGGAGGATTGGAGCCCATCCCACCAGTA25600.10204020623282306No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT25520.1017213305883455No Hit
GATTACATATATCACAAAAAATCAACCTGAGTGGTTCAGAAACATCCTGA25490.10160175222166641No Hit
GTTATTAGATCTCCTTTTGCAAGCAGAGCTTGTTCCATTTTGAGTGACTC25430.10136259548830823No Hit
ACATACAATTGATCTAACTGACTCAGAAATGAACAAACTGTTTGAAAGAA25400.10124301712162913No Hit
CTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTT25390.10120315766606945No Hit
ACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATA25350.10104371984383065No Hit
TCTCTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCA25220.1005255469215546No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGGG353.433362E-582.881111
TTCGGAC851.8189894E-1276.763953
GCACCGA300.00193600772.500725145
TCGGACA1009.094947E-1265.249354
CGGACAT1102.5465852E-1159.3175965
CTTCTAA22350.054.5123981
GTGTATA400.006045028654.390721
GACATCT1258.913048E-1152.1994867
CATATAC12900.051.1429063
TACTGAT12900.051.1429067
TAGATGG24200.050.6296658
TTCTAAT24650.049.4132462
ACTGATG13500.048.869898
ACTCTGG1551.4551915E-1146.787721
ACAGGGC657.328264E-444.6282841
TTTCGGA1755.2750693E-1141.4298132
GGACTCT1755.2750693E-1141.428166
TATACTG29600.038.2090845
CTTTCGG1901.2914825E-1038.1689261
ATACATC37800.037.2086263
Produced by FastQC (version 0.11.5)