FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n01_TR_DEMUXING_CONTROL_P1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n01_TR_DEMUXING_CONTROL_P1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA120.7125890736342043No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG110.6532066508313539No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA70.4156769596199525No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT70.4156769596199525No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT60.35629453681710216No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA50.29691211401425177No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA50.29691211401425177No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC50.29691211401425177No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA40.23752969121140144No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT40.23752969121140144No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA40.23752969121140144No Hit
GAAGAACACTGATCTTGAGGTTCTCATGGAATGGCTAAAGACAAGACCAA40.23752969121140144No Hit
GCCTTAATGAGCTGCCCTGTCGGTGAAGCTCCGTCCCCGTACAATTCAAG40.23752969121140144No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT40.23752969121140144No Hit
AGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAAA30.17814726840855108No Hit
CTGTATTCCCATTGAATGCTGCCATAATGGTTGTTCTGTCAAAAGGGAGA30.17814726840855108No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG30.17814726840855108No Hit
CTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAATGTTATTT30.17814726840855108No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT30.17814726840855108No Hit
GGTCCATGCTCATACCCAAGCAGAAAGTGGCAGGCCCTCTTTGTATCAGA30.17814726840855108No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT30.17814726840855108No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT30.17814726840855108No Hit
CTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTG30.17814726840855108No Hit
GTTCTTATCCATGATCGCCTGGTCCATTCTGATACAAAGAGGGCCTGCCA30.17814726840855108No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC30.17814726840855108No Hit
TTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTCATG30.17814726840855108No Hit
GTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTTGA30.17814726840855108No Hit
ATTGAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTACTCTCTATC30.17814726840855108No Hit
GGCTACGGCAGGTCCATACACACAGGCAGGCAGGCAGGACTTGTGAGCAA30.17814726840855108No Hit
GCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATCTTCAGTTTGTGT20.11876484560570072No Hit
GACAAAGACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTG20.11876484560570072No Hit
GGTGAACACTGTTATCGAGAAAATGAACATTCAATTCACAGCTGTGGGTA20.11876484560570072No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA20.11876484560570072No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG20.11876484560570072No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT20.11876484560570072No Hit
GTCTTTCTTTTGTTCCAAGATCACATCCAGGATGCCACATTATATTCAGT20.11876484560570072No Hit
ATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACAGTAC20.11876484560570072No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT20.11876484560570072No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG20.11876484560570072No Hit
GGCTATGCAGCGGATCAAAAAAGCACACAAAATGCCATTAACGGGATTAC20.11876484560570072No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT20.11876484560570072No Hit
GAGCGAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG20.11876484560570072No Hit
GAGCGAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT20.11876484560570072No Hit
ATCCACAGCACTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTG20.11876484560570072No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA20.11876484560570072No Hit
ATTACCGCTTGCCCGACTGGAATCGCTCACACGTATATGGCGGCGGAAGC20.11876484560570072No Hit
GTCCCCGTACAATTCAAGATTTGAATCGGTTGCTTGGTCAGCAAGTGCAT20.11876484560570072No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA20.11876484560570072No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA20.11876484560570072No Hit
TCCACATTTTCTCGTTCACTGGGGAAGAAATGGCCACAAAGGCAGACTAC20.11876484560570072No Hit
ATTCCTCATAGTGAGAATTAGGTGCATTCAACTCTATTGATTTAGTAACC20.11876484560570072No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG20.11876484560570072No Hit
TTCTGATACAAAGAGGGCCTGCCACTTTCTGCTTGGGTATGAGCATGGAC20.11876484560570072No Hit
GTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGT20.11876484560570072No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT20.11876484560570072No Hit
GAGTAGAGGCTTTGGGTCCGGCATCATCACCTCAAACGCATCAATGCATG20.11876484560570072No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA20.11876484560570072No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA20.11876484560570072No Hit
CCATACAACTGGCAAGTGCACCAGCAGAATAACTGAGTGAGATTTCTTTG20.11876484560570072No Hit
CATTCCATGGGGCCAAAGAAATCTCACTCAGTTATTCTGCTGGTGCACTT20.11876484560570072No Hit
CAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGTATGTG20.11876484560570072No Hit
ATTCTCTGTTATCTTCAGTTTGTGTCTCACTTCTTCAATCAACCATCTTA20.11876484560570072No Hit
GCCACTTCAGTGGTCACAGCCCCCATCCTGTTGTATATGAGGCCCATACA20.11876484560570072No Hit
TCTCAGAGTTTGGTGTTTCTACAATGTAGGACCATGATCTCACTGGAAGC20.11876484560570072No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC20.11876484560570072No Hit
GCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCA20.11876484560570072No Hit
CTAGCAACCTCCATGGCCTCTGCTGCTTGCTCACTCGATCCAGCCATTTG20.11876484560570072No Hit
AGCGAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGGC20.11876484560570072No Hit
GGTTGCACATACCAGGCCAAATGCCACTTCAGTGGTCACAGCCCCCATCC20.11876484560570072No Hit
CCTATCGACAATGTGATGGGAATGATTGGGATATTGCCAGACATGACTCC20.11876484560570072No Hit
GGAAAGGGCATGTGTCAAAGGTCTGGCCAATCAGAGAGCCATATCATCTG20.11876484560570072No Hit
GGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGTATGTGCAACC20.11876484560570072No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT20.11876484560570072No Hit
GGACTGCAGCGTAGACGCTTTGTCCAAAATGCCCTTAATGGGAACGGGGA20.11876484560570072No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG20.11876484560570072No Hit
ACATTCCATGGGGCCAAAGAAATCTCACTCAGTTATTCTGCTGGTGCACT20.11876484560570072No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG20.11876484560570072No Hit
CCATTAGGATCCCAAATCATCTCAAACCCATGTCTGGAACTGTGACTTTT20.11876484560570072No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC20.11876484560570072No Hit
GTGAAAGCCCTTAGCAATATTAGAGTCTCCAGCCGGTCAAAAATCACACT20.11876484560570072No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA20.11876484560570072No Hit
ACAATAGAGAGAATGGTGCTCTCTGCTTTTGACGAAAGGAGAAATAAATA20.11876484560570072No Hit
CTTGAATACAAGTCAAAGAGGAGTACTTGAAGATGAACAAATGTACCAAA20.11876484560570072No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA20.11876484560570072No Hit
CTCTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT20.11876484560570072No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT20.11876484560570072No Hit
TCTCTGTAGAGTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTC20.11876484560570072No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA20.11876484560570072No Hit
AGGCTATGGAGCAAATGGCTGGATCGAGTGAGCAAGCAGCAGAGGCCATG20.11876484560570072No Hit
TCTCATTACTGCTTCTCCAAGCGAATCTCTGTAGAGTTTCAGAGACTCGA20.11876484560570072No Hit
ATGAGGAGCTGAGGGAGCAATTGAGCTCAGTGTCATCATTCGAAAGATTC20.11876484560570072No Hit
GGGCATCTGCGGGCCAAATCAGCATACAACCTACGTTCTCAGTACAGAGA20.11876484560570072No Hit
GTCAAGTGCAAGATCCCAATGATATTTGCGGCAATAGCGAGAGGATCACT20.11876484560570072No Hit
GTATGGGCCTCATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCA20.11876484560570072No Hit
ATGTAACATCGCCGGATGGCTCTTGGGAAACCCAGAATGCGACCCACTGC20.11876484560570072No Hit
ACCCACACGCTGGCGGCGCAGCGGCTGACGCTCGGGTACGGCGAGCGAAC20.11876484560570072No Hit
GAATATACACCCAGTCACAATAGGAGAGTGCCCAAAATACGTCAGGAGTG20.11876484560570072No Hit
GTAGAAACAAGGGTATTTTTCTTTAATTGTCGTACTCCTCTGCATTGTCT20.11876484560570072No Hit
GTCATGGAAGCAAGTACTGGCAGAACTGCAGGACATTGAGAATGAGGAGA20.11876484560570072No Hit
TTCCTATCCAAACACCATTACCATACCTATATGAAAATCCCTTTACTCCG20.11876484560570072No Hit
ATAAGAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGA20.11876484560570072No Hit
CCTTCTCCGTCAGCCATAGCAAATTTCTGTAAAAACTGCTTTTCCCCTCA20.11876484560570072No Hit
GATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAAG20.11876484560570072No Hit
GTATTTAAAGCGACGGTAAATGCATTTGAAAAAAAGACGATCAAGAATCC20.11876484560570072No Hit
TCCCCATACTCTTTCATTGTTTTTTCCGCAAGCTCGACAATCATCGGATT20.11876484560570072No Hit
GATTAATTCAACCCAGAAGCACGGCCTCATACAGTCTAGCCCTGTTAGCT20.11876484560570072No Hit
TTCTCATGGAATGGCTAAAGACAAGACCAATCCTGTCACCTCTGACTAAG20.11876484560570072No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG20.11876484560570072No Hit
GTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGTATGATGTT20.11876484560570072No Hit
ATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACAGT20.11876484560570072No Hit
GCACTGGAGCTAGGATGAGTCCCAATGGTTCTCATCGCTTGCACCATTTG20.11876484560570072No Hit
AGCCTTAGCTGTAGTGCTGGCTAAAACCATTCTGTTCTCATGTCTGATTA20.11876484560570072No Hit
TTTCCAGATAGTATATGTGAACTTCTCTCCTTGTTACTCCAATTTCGATG20.11876484560570072No Hit
GTCCTACACCGGCACCGGCGTGCTCTTCGCGGCGCTCATCTCCGGCCTGA20.11876484560570072No Hit
ACCCTGACCAATCAGTCATTGCCACAACATCTTGCCTAACAGAGAACTTA20.11876484560570072No Hit
CTTCAAGCAATAGATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTA20.11876484560570072No Hit
GAACAGCGGACTCCACTCCAGCTGTGCCTTCATCTGGGTCTTCAGTTAAA20.11876484560570072No Hit
CCTCAGCAGTATGTCCTGGAAGAGAAGGCAATGGTGAAATTTCGCCAACA20.11876484560570072No Hit
CAATATGAGTGCAGACCGTGCTAGAAAAGTGAGATCTTCGAACTCAGCAT20.11876484560570072No Hit
AGACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTC20.11876484560570072No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)