FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n01_TR_CW_E1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n01_TR_CW_E1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218778
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA201761.6554286342549667No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG83690.6866714036518545No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA76610.6285804305624157No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG61880.5077216687534563No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA55980.45931252451225735No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA55170.45266652335371993No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG48840.4007292550407047No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG44470.3648736685434099No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA38960.3196644507859512No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG32690.2682194788550499No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT30930.2537787849797092No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT30780.2525480440244245No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT30700.2518916488482726No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC28790.2362202140176472No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA28600.23466127547428656No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC27280.223830755067781No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT25800.21168744430897177No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA25120.20610808531168107No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA24540.20134922028458013No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGT22270.18272400716127138No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT21590.17714464816398065No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT21520.17657030238484778No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGC21290.1746831662534112No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT20800.17066274579948112No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT20580.16885765906506353No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT19880.16311420127373485No Hit
GCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGA18710.153514421822514No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT18480.15162728569107745No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT18330.15039654473579273No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC18190.149247853177527No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG18150.14891965558945106No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT17830.14629407488484367No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG17490.1435043953861983No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG17480.14342234598917933No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG17380.1426018520189895No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT17040.13981217252034417No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT17030.13973012312332517No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA16650.13661224603660388No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA16350.13415076412603444No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT16230.13316617136180667No Hit
GTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGATATTT16130.13234567739161684No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC16060.13177133161248397No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT15800.12963804728999048No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC15600.12799705934961084No Hit
GTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGAGTG15380.12619197261519324No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA15250.1251253304539465No Hit
ATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGCCC15000.12307409552847196No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC14670.12036646542684558No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC14580.11962802085367474No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT14540.11929982326559882No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG14310.11741268713416225No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACC14240.1168383413550294No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAAAC14060.11536145220868772No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA13790.11314611848917522No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC13780.11306406909215624No Hit
TCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTTCCGT13430.11019234019649189No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA13230.10855135225611226No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA12890.10576167275746691No Hit
GTATTAGGCGTCTCCATCCTGAATCTTGGACAAAAGAGATACACCAAGAC12810.10510527758131505No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA12610.10346428964093542No Hit
CTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTG12480.10239764747968867No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT12450.10215149928863172No Hit
TCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCCA12410.10182330170055581No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACA12190.1000182149661382No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAT100.007080769145.070391
GATAGCC100.0070912014144.998983
GAGCGAA9850.0143.59761
TTCTCTA256.4498636E-6116.0563051
TCCCCCC404.816684E-9108.749239
AGCAAAA69300.0106.504332
ATTAGTA1650.0105.505741
GCAAAAG70300.0104.572513
CTCCCCC451.0919393E-896.6659858
CGCTCCC451.0919393E-896.6659856
GAGCAAA80500.092.178271
AGCAGGC3000.091.832688
GCAGGGT25650.087.903869
CAAAAGC86450.085.3724444
AAGCAGG105300.083.240157
GCAGGGG9650.081.139339
AGCAGGG38350.079.5887458
GCAGGTC54650.075.351719
AGCAGGT74200.073.574288
AGCGAAA20450.073.4219361
Produced by FastQC (version 0.11.5)