FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n01_TR_CW_B4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n01_TR_CW_B4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4352945
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG213830.4912306495946997No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA132250.30381730070101964No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG113810.26145517574883215No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT99600.22881060982851842No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA82850.19033091389852158No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT82500.1895268605507306No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC79930.18362281168266542No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT74060.170137688392571No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA69160.15888094152349733No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG68120.1564917544329184No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT66870.15362013533366492No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT65780.15111608347911587No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA63350.14553365595016707No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT60750.1395606882237198No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT60640.1393079857429855No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT59520.13673501503005436No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACA56800.13048637187007875No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG55630.12779853639317748No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT54790.12586880835847913No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA54130.1243525934740733No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA52850.12141205551643773No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC52150.11980394882085577No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA51140.11748368058865895No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT48700.11187828010691611No Hit
CTGTTAACCTGCTCGAAGACAGCCACAACGGAAAACTATGTAGATTAAAA47500.10912152577163277No Hit
GAGTAGAGGCTTTGGGTCCGGCATCATCACCTCAAACGCATCAATGCATG47390.10886882329089846No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA47330.10873098557413428No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT46230.10620396076679121No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA45910.10546882627738233No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA44970.1033093687147437No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA44110.1013336947744573No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT43980.10103504638813493No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCAGG100.007078526145.090031
CTATAGG100.007091913144.998354
GAGCGAA15450.0138.98591
AGCAGGC7650.0108.9856958
GCAAAAG108250.0105.282873
ATTAGTA3750.096.7266851
GCAGGGG34550.087.502639
CTCCTAC259.387587E-487.003006145
ACTAATA2250.086.999018
TACTAAT2300.085.107737
GCAGGGT68200.082.066519
AGCGAAA35100.081.432291
AGCAAAA146200.078.453472
TTAGTAG4650.077.9596942
AGCAGGG116450.074.024498
AGCGAAG300.001935913872.502512
CTACTAA2700.072.499176
AAGCAGG188950.071.559137
TCTACTA2850.068.683435
CAAAAGC169100.067.6544654
Produced by FastQC (version 0.11.5)