FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n01_TR_740-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n01_TR_740-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022270
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG13540.13245033112582782No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC11880.11621195965840726No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG11220.10975573967738465No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC10710.10476684241932172No Hit
CCATCTCAACAGATGCAGAAAAAGCATTTGATAGCGCTGCTACCAAATCA10360.10134308939908243No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTG2300.063.04292
GAGCGAA4950.061.5507241
GTTACGT2550.059.7404021
TACGTGT2550.056.862233
GAGCAAA27100.054.60721
AGCGAAA6800.050.1392671
CGAAAGC6950.049.028333
GCGAAAG7500.045.4329222
GACGACT2200.042.840524
AGACGAC2250.041.8885083
GGGTTAC2300.041.001951
ACGTGTT3600.040.277414
CAAAAGC37850.038.88344
GCATGTT9050.038.4526864
GTAGCAT9950.037.9113541
GCAAAAG38700.037.6546943
CGTGTTG3950.036.7085275
ACTCCGA2201.6370905E-1136.2709581
TATGCGT800.002036727536.249676
AGCAAAA40800.035.7165872
Produced by FastQC (version 0.11.5)