FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n01_TR_644-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n01_TR_644-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977665
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA29850.066.832771
GAGCGAA5550.061.43361
GCGAAAG8250.059.757092
AGCGAAA8450.058.378551
GCAAAAG35150.056.7207343
ACTCCGA1850.054.8981131
CAAAAGC36750.054.448544
AGCAAAA38700.051.1429942
CGAAAGC10400.047.403463
AAAGCAG56100.041.354396
AAAAGCA49650.040.1557665
CCGAGGT2550.039.80364
CGAGGTG2750.036.9087945
TCCGAGG2800.036.2497023
AAGCAGG73850.029.5495267
CCGACTA1409.994906E-425.8926454
GTTACGT1701.027032E-425.6037431
GTATAGG3451.07320375E-1025.2326721
GCAGGGG27250.023.944769
TATATAG6000.019.3331785
Produced by FastQC (version 0.11.5)