FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n01_TR_562-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n01_TR_562-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1554143
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG37240.2396175898871597No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA26620.171284109634699No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC19970.1284952542977062No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT16650.10713299870089175No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC16120.10372275910260509No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4400.0110.4781341
GTTACGT1250.069.650691
ACTCCGA2200.065.957091
AGCGAAA7700.062.14412
GCAGGTA9800.059.182959
TTACGTG1550.056.1301572
TCCGAGG3000.055.5826573
GCGAAAG8650.055.3172453
GTCGTCT1051.1095835E-955.239197145
CGAAAGC8800.054.3743364
GCGATTA1101.6752892E-952.728325145
TACGTGT1650.052.726633
GAGCAAA48250.051.4260251
CGAGGTG3400.049.0435185
AAGCAGG65400.045.2288067
GCAAAAG58150.042.514533
CCGACTA2650.041.0372354
CAAAAGC60750.040.8143164
GTTATGT4800.040.810951
GTAGAAA11450.039.919881
Produced by FastQC (version 0.11.5)