FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n01_TR_559-3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n01_TR_559-3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1289997
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC16030.12426385487718189No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA37250.059.7809221
CGAAAGC11150.058.519323
GCGAAAG11500.056.7382932
GAGCGAA10650.051.7624131
GTTACGT1600.049.8681451
AGCGAAA13200.048.90651
GCAAAAG47100.046.0237433
CAAAAGC48700.044.8094024
TTACGTG1801.8189894E-1244.3049052
AGCAAAA50050.043.4559062
TACGTGT2200.039.544873
AAAGCAG69200.039.287726
GTAACGG2500.037.718451
GGTAACG800.002036980436.249472
TAACGGG2600.036.249472
GGTGCGA2154.8748916E-1033.7204368
ACGTGTT2603.6379788E-1233.461054
CGGGTTG2850.033.069695
AACGGGT2850.033.069693
CGGTTAC1102.4295501E-432.9706731
Produced by FastQC (version 0.11.5)