FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n01_TR_545-3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n01_TR_545-3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954581
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA3600.086.646331
AGCGAAA9050.083.3621751
GCGAAAG9300.080.2949452
CGAAAGC10100.073.934953
GAGCAAA18750.049.1345141
CGTACAC700.001056665641.4281853
CAAAAGC22850.040.9295964
GCAAAAG22850.040.295033
CAGCGCG750.001483421138.66639
AGCAAAA25200.036.5373572
AGTAGCG1000.00607459928.9997275
GAAAGCA28550.027.6792494
AAAGCAG46500.026.9728666
AAAAGCA35950.026.6200565
TATGCGT1550.001808528123.3868774
GTATAAT7250.023.0130441
AAGCAGG58050.020.482157
CCTATAC4301.7007551E-920.2323683
GTGTAGT3259.775413E-720.088311
GTACTAA4001.6207196E-819.9488091
Produced by FastQC (version 0.11.5)