FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n01_TR_528-3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n01_TR_528-3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194139
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8100.079.719341
GAGCAAA35950.062.9672051
AGCGAAA11550.057.1634061
GCGAAAG12050.055.3518332
CAAAAGC41600.053.3284954
GCAAAAG43900.050.864813
CGAAAGC13200.049.980283
AGCAAAA46550.047.8134352
GTTACGT2150.043.869591
AAAGCAG66000.043.609176
AAGCAGG76800.036.1550377
AAAAGCA64000.035.003365
TTACGTG2700.034.9068642
TACGTGT3403.6379788E-1227.7201563
GCAGGGG31300.026.6369639
ACTCCGA2301.0963358E-625.236021
CGAAGCG2054.3746695E-924.755719
CGAGTAA1500.001494578224.166294
AGACGAC2101.5555846E-524.166293
ACGTGTT3652.2009772E-1023.8352454
Produced by FastQC (version 0.11.5)