FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n01_TR_286-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n01_TR_286-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1037047
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC24280.23412632214354798No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG17760.17125549758111253No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA16070.1549592255702972No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA15420.14869142864306054No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA14260.13750582181906895No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC13780.13287729485741726No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA12560.12111312216321922No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG10580.10202044844640601No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATT353.4376084E-582.855838
GAGCGAA4950.082.070341
GTCGTCT1250.075.406075145
TTACGTG2250.074.109932
ACGGTAT406.663831E-572.498847
GTTACGT2350.071.0008241
TACGTGT2400.069.478063
AGCGAAA6700.059.5139732
CGAAAGC6800.058.6387674
GCGAAAG7000.057.9990733
GAGCAAA30150.054.8593831
ACGTGTT3050.054.6712574
AATCCGC400.006036339354.4082341
ACTCCGA4200.050.0901221
AATGGGT8650.049.4500777
TCCGAGG4350.048.332563
GGGTTAC2100.041.453891
CAAAAGC41000.040.1396034
AAGCAGG51650.039.863847
AACGGGT2200.039.5448233
Produced by FastQC (version 0.11.5)