FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n01_TR_283-5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n01_TR_283-5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208316
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG33930.2808040280853684No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA22340.18488541077003035No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC19390.16047126744990547No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG18150.15020905127466655No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC16140.13357432989383572No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA15310.12670526584105482No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA15000.1241397117972451No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA14850.12289831467927263No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA13420.11106366215460195No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC12960.10725671099281976No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG12580.10411183829395622No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC12190.10088420578722784No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12190.10088420578722784No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4200.0124.346651
GTTACGT2650.090.327281
TTACGTG2650.090.2824252
TACGTGT2750.086.9994353
GTCGTCT554.395406E-879.090385145
AGCGAAA6800.076.76422
GCAGGTA7550.072.0193949
GCGAAAG8400.064.731713
GGTTATA2650.062.9553761
CGAAAGC8350.062.5145574
GGTCGTT902.050183E-856.3885157
GTCGAAG1800.056.3885153
CGAGTGT400.00605134654.3746458
CTTTCGG400.00605134654.3746452
GAGCAAA37100.053.570731
AGCAGGT14650.053.446758
GTAACTC1258.913048E-1152.1996573
GCGAAAT450.00963986948.3330152
AATGGGT8700.048.3330157
TAAATCG450.00963986948.3330155
Produced by FastQC (version 0.11.5)