FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n01_TR_283-3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n01_TR_283-3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2731926
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG111140.40681921838292834No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA86120.31523547855981454No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA75050.27471461525678226No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT69310.25370379724780245No Hit
ATTCAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATG56200.20571567458269369No Hit
GGATACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAGG45850.16783031458392358No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA43450.15904530356971602No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG40560.14846668614010775No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA39470.14447682697115513No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC38870.14228057421760326No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG36250.13269027052709334No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC33720.12342940474961621No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT33520.12269732049843224No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC32800.12006181719416997No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT31410.11497383164844144No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT30070.11006886716550889No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA30030.10992245031527209No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC28960.1060057995714379No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT28610.10472465213186594No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC27470.10055177190011735No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG27370.10018572977452536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA11050.0135.23951
GTTACGT2700.085.977511
TTACGTG3050.076.066662
TACGTGT3050.076.063873
GAGCAAA106000.073.364771
GCAGGTA21500.073.172789
AGCGAAA21100.070.7832
AAGCAGG137550.067.517587
GCGAAAG23150.065.765273
CGAAAGC22750.065.646884
AGCAGGT56250.058.772028
ATGTCGA753.748064E-758.034821
AGTCGGC1800.056.3876275
AGCAAAA138150.056.1535382
AAAGCAG166050.055.754556
CACCTAC3400.055.4399383
AGCTACT3150.055.236868
CAAAAGC142650.054.5326044
CGTTGTT400.006052538354.3737836
ATGGGTG13200.052.7260938
Produced by FastQC (version 0.11.5)