FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n01_TR_199-7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n01_TR_199-7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920770
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA17350.18842924943254016No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG14520.15769410384786645No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA14100.1531327041497877No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA12780.13879687652725436No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC11630.12630732973489578No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC11220.12185453479153316No Hit
CCATCTCAACAGATGCAGAAAAAGCATTTGATAGCGCTGCTACCAAATCA9490.10306591222563723No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1850.0109.78841
AGTCGGC1300.078.076255
CTCGCAA651.3980207E-766.92253
AGCGAAA4500.064.4789051
TACGTGT1350.059.0735663
CGAAAGC5050.058.860883
TTACGTG1400.056.9637952
GCGAAAG5400.055.0458222
ACCGAAC400.006050939754.3745358
GTTACGT1353.6379788E-1253.7324181
CGAAAGT701.6959662E-551.7852674
GGTTATA1457.2759576E-1250.0267331
TATGTGT5100.049.7544753
CAGTCGG2050.049.511774
CGGCTGG2550.048.332928
AGCTACT3300.048.3329168
ACGGTCC450.00963922448.3329163
ACTCCGA2300.047.307891
GAGCAAA23550.046.8190771
AGTCCGC1551.4551915E-1146.77633145
Produced by FastQC (version 0.11.5)