FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n01_TR_197-7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n01_TR_197-7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1273345
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG26380.20717087670662707No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA18910.14850649274155864No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC17490.1373547624563649No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG17210.13515582972407322No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA16530.12981556451707904No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC14120.11088903635699673No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA13880.10900423687217527No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2250.0132.181881
GTCGTCT703.092282E-1182.85921145
AGCGAAA5200.075.328791
TACGTGT2050.070.730693
GTTACGT2200.065.94441
TTACGTG2200.065.908142
CGAAAGC6100.062.99093
GCGAAAG6100.062.99092
GCGATTA1450.060.001495145
GAGCAAA38650.055.3659931
ACGGGTT3450.054.6368984
AGTCGGC1900.053.4202845
GCAGGTA8300.052.4088869
AACGGGT3800.049.604553
AAGCAGG50750.048.9992947
GTTACGC450.00961936448.3592221
GCGACTA1203.623427E-948.3326384
AGTCGTG2150.047.2086262
GAAGGCG2000.047.1243254
ATGTCGA1255.173206E-946.4248541
Produced by FastQC (version 0.11.5)