FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n01_TR_156-7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n01_TR_156-7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1546677
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG29190.18872718738301533No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC24930.16118426794993396No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA24010.15523603182823562No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC22950.1483826293401919No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA22160.14327490484438574No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA21830.14114129840942874No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG21140.136680121318155No Hit
CCATCTCAACAGATGCAGAAAAAGCATTTGATAGCGCTGCTACCAAATCA20420.13202498000552151No Hit
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC20270.13105515889872285No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT17770.1148914737854122No Hit
AGTCAGTGGAGCTCTGTGACTCCTGATCTTGTGGTTTTGAGTTTCAACCC16150.1044174058319869No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG15580.10073208562615206No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2300.091.450461
GTTACGT3800.070.6211
TACGTGT3800.068.683453
TTACGTG4050.066.233832
GTCGTCT1600.058.9094145
CTCGCAA902.0507287E-856.388263
TCGCAAA1004.7059075E-850.7494354
AGCGAAA4700.049.3819051
ACGTGTT5400.048.3327984
CGAAAGC4800.048.3327943
CATTCTA18950.046.6749422
CAGCAAC7150.045.628867
ACTCCGA4700.044.752351
TCCGAGG6050.043.140023
GCGAAAG5650.042.344662
GGACCGT1053.8147336E-641.445531
CTACTGA25300.040.9778026
TATGTGT11650.039.827883
GAGCAAA40100.039.7918431
TTATGTG11700.039.6576772
Produced by FastQC (version 0.11.5)