FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n01_TR_123-7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n01_TR_123-7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2282898
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATGAATGGGTGGGTGGAAGGGACAGCGGTGGGTGTGTGGGGTGCAGC38650.16930235166003912No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG36000.15769429908826413No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA35330.15475943296634365No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA31750.1390776110014552No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA31470.13785109978632423No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC30950.1355732932439382No Hit
CCCTGTGCCATCCCCCACTGCCACCCTGCTGCACCCCACACACCCACCGC29580.12957214908419035No Hit
CCATCTCAACAGATGCAGAAAAAGCATTTGATAGCGCTGCTACCAAATCA25130.11007938155800216No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG23710.10385921753840952No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6050.0104.31721
TACGTGT5550.077.071563
TTACGTG5600.076.383422
GTTACGT5650.075.752261
AGCGAAA10900.057.8665922
CGAAAGC11250.056.7107354
GCGAAAG12600.055.813123
AGTCGGC2700.053.7033425
ACTCCGA6600.052.758121
GGTTATA4450.052.1653331
ACGTGTT8550.051.7248044
TATGTGT14850.048.8212243
AATGGGT16500.046.5754477
GATATAC6500.045.7304653
GAGCAAA62350.045.6080631
TCCGAGG7600.044.8352283
GTTATGT17550.042.574751
AACGGGT7100.040.84483
ATGGGTG18300.040.805748
CAGCAAC11600.040.624737