FastQCFastQC Report
Wed 21 Nov 2018
HNJT3BCX2_l01_n01_TR_122-3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJT3BCX2_l01_n01_TR_122-3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1708417
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG77520.4537533869072949No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA71920.4209745044681714No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA39930.2337251385346786No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT39060.22863270501288618No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC38450.22506214817576742No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT36040.21095552198321602No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC33900.19842930619397955No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT32420.18976631583506837No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT30100.17618649311028867No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT29580.17314273974094146No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA27710.16219693435501986No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC27570.1613774622940418No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT26980.15792397289420557No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGG26380.15441194977572806No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA24960.1461001617286646No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG24910.14580749313545815No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC24510.14346614438980645No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG24100.1410662619255135No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG23460.13732010393247082No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC22720.13298860875301521No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT21760.1273693717634512No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC21740.1272523043261686No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC21610.12649136598383182No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGC21470.12567189392285374No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC21020.12303787658399559No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA20990.12286227542807172No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA20460.11975998834008325No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT20190.11817957793676837No Hit
CATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAG19000.11121406541845463No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC18790.1099848573269875No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT18050.1056533621475319No Hit
AGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACC17960.10512655867976027No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT17960.10512655867976027No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG17950.10506802496111899No Hit
TTCCATGAGAGCCTCAAGATCTGTGTTCTTTCCTGCAAAGACACTTTCCA17910.10483389008655382No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC17790.1041314854628583No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC17730.10378028315101055No Hit
AAATGGGAATGGGGACCCGAACAACATGGATAGAGCAGTTAAACTATACA17730.10378028315101055No Hit
TTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCCC17630.10319494596459763No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGT17410.10190720415448921No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA5550.0120.229071
GCAGGTA14750.087.982799
AGCGAAA11650.072.2182161
GCAGGGT27900.068.342099
GCGAAAG12650.066.4839552
CGAAAGC12900.065.19363
GAGCAAA60650.063.1419221
AAGCAGG77200.057.3800057
GCAAAAG67000.056.9177363
AGCAAAA69200.055.1098252
AGCAGGT28400.053.098438
GTAGAAA14050.051.6224171
AGCAGGG47900.049.342238
AAAGCAG104550.042.924316
CAAAAGC89200.042.8333934
CTACACT8300.042.801084
TCTACAC8500.042.646933
TAGAAAC18250.040.918882
GGTAGTT8600.040.4649937
CATACTC7800.039.9678273
Produced by FastQC (version 0.11.5)