FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10898346
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC122450.069.843262
TAACAAG125450.067.908685
AAACACG56050.060.7967535
GCACGCA21900.054.5742452
GTAACAA178050.047.9273344
CACGAGC50550.047.329268
CACGCAC25950.045.783193
ACACGAG53350.045.2268527
CGCACTT25200.045.1695335
AACACGT23650.041.471566
ACGCACT31200.040.1543354
ACGAGCC18600.039.6811689
GTAGTAA240250.037.152861
TCTCGGG30750.036.913322145
ACACGTG27300.035.667877
ACAAGAG264400.032.3599137
ACAACGC31300.030.2088388
AGTAACA305100.028.3843213
AACAAGA307850.027.8598426
GTAGAAA175450.026.0289421