FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_POSITIVE_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_POSITIVE_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2599545
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT108930.41903486956371205No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT99710.38356712424674316No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC93450.3594859869707968No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT65960.25373671161684064No Hit
GTACTTCCTTCATTGTTTCTTTGGCCTGGATTTCTTTTTGGTAACTGTGT58730.22592415211123484No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT54860.21103693146300603No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT48030.18476310277375463No Hit
GTATAATGGAGACCCATGGGCTGACAGTGATGCCCTAGCTTTTAGTGGAG45260.17410739187050042No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT41290.158835488518183No Hit
GTTTTCATAACCTCTTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA40900.15733522597223745No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC40830.15706594807937543No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT39330.1512957075180464No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG37290.143448180354639No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT34060.1310229290125772No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT31530.12129045659913561No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG30480.11725128820620531No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT30190.11613570836434839No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT28750.11059627742547253No Hit
CCATTATACTTGACATACAGTCCCATCCCCATCCTTTCAGTCTTAGACAT27820.10701872827744854No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA27320.10509531475700554No Hit
CCCCCAGACCAGATGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG27270.10490297340496124No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT27130.10436441761923722No Hit
GTATTATTATCACTATATGTGTCAGCTTTATTATCATACTTACTATATTC26960.1037104570222866No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT26110.1004406540375335No Hit
AGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATTGTTCAAACA26000.10001750306303603No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA55350.0129.115464
AGCACGC15900.0121.284081
CACGCAC16450.0117.649323
GCACGCA16450.0117.210942
GAAACAC21250.0112.898464
AAACACG21600.0110.395855
ACCCGCC404.813046E-9108.766235145
TAACAAG68500.0104.432865
GAGCCCC1400.0103.5869145
TAGAAAC26500.098.1976242
ACGCACT21200.091.625834
GAAGCGG6200.091.2057341
GCCGACC1750.091.15647145
CGCACTT21100.090.6842965
TAGTAAC79300.090.675692
TCTGACC1850.086.22909145
GAGCAGA3350.084.3993451
ACGTGCA9900.081.271862
AGTAACA89800.079.991053
AACACGT5550.079.649056
Produced by FastQC (version 0.11.5)