FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_PLASMID_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_PLASMID_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2053656
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT53150.2588067329679362No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG49840.24268913586306567No Hit
CTTCTAATAGATGGCACAGCATCATTGAGCCCTGGGATGATGATGGGCAT47520.23139220979560357No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG43630.21245038117386747No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT34560.16828524348771165No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA34420.16760353243191656No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT33200.16166290751713042No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG33130.16132205198923286No Hit
GATGTATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGT32450.15801088400394223No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC29100.14169851231170166No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT29080.14160112501801664No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGGTACA27980.13624482386534062No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA27430.13356667328900262No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT25730.12528875332577608No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG25410.12373055662681577No Hit
GTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA24240.1180333999462422No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT23760.11569610489780179No Hit
ACATATAGGTGCCATAGAGGAGACACACAAATTCAGACGAGAAGATCATT23420.11404052090515646No Hit
CCTATATGTATATCTGTAGTCTTTGATGAACAATTGGAGAGCCATCTGGG23300.11345619714304635No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTTT23230.11311534161514879No Hit
TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC23110.11253101785303866No Hit
ATCTGGGACAGGCCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTG22650.11029111009828325No Hit
ATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG22640.11024241645144074No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT22480.1094633181019606No Hit
ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT21250.10347399954033197No Hit
ATGTATATCTGTAGTCTTTGATGAACAATTGGAGAGCCATCTGGGCTGTT20630.1004549934360964No Hit
ATTGTATGTTGGTTCTCCAGGGCAACAAGAAGCTCCGCGTTGTATGACCA20620.1004062997892539No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCCC2250.058.006813145
CGACGAA9250.051.73581145
ACCTACC1004.7022695E-850.755966145
AGGACCC901.3231911E-648.339012145
GGGATCG752.555274E-548.3213465
AGTGCGT450.0096528148.3178148
GCCGACC2800.046.612617145
GTTCGGG951.9203253E-645.794853145
TAGATGG19350.045.6959158
GGATCGG803.743668E-545.296856
CTTCTAA19800.043.93921
TGGACCC1505.366019E-1043.50511145
CGGACAT1002.7402275E-643.4892125
TAACTAA855.3397634E-542.65207145
TTCTAAT21100.041.5675432
TACTGAT11500.040.334877
CATATAC11700.040.2697533
TCTGACC3150.039.131584145
CGTCAAA2450.038.47391145
CCGTTCC1701.8299033E-938.386864145
Produced by FastQC (version 0.11.5)