FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_NEGATIVE_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_NEGATIVE_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7813
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCGGGTTATTAGTAGAAACAACGCACCGGGTTATTAG550.7039549468833995No Hit
GTAGTAACAAGAGCGGGGGGAGCAGAAGCACGCACTTGGGGGGAGCAGAA370.47356969153974143No Hit
ATTAGTAGTAACAAGAGCCGGGTTATTAGTAGAAACAACGCACCGGGTTA230.2943811596057852No Hit
GTAGTAACAAGAGCTCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATT200.2559836170485089No Hit
GTAGAAACACGAGCGTGCTTCTGCTCCCCCCGCTCCGCTTCCTGTCTCTT180.230385255343658No Hit
CTTCTGCTCCCCCCGCTCCGCTTCTGCTCCTGTCTCTTATACACATCTGA160.20478689363880712No Hit
GAGCAGAAGCGGAGCGGGGGGAGCAGAAGCTGTCTCTTATACACATCTGA160.20478689363880712No Hit
GTAGAAACACGTGCCTGCTTCTGCTCCCCCCGCTCCGCTTCCTGTCTCTT150.19198771278638166No Hit
GTAGAAACACGTGCGTGCTTCTGCTCCCCCCGCTCCGCTTCCTGTCTCTT150.19198771278638166No Hit
GAAGCGGAGCGGGGGGAGCAGAAGCACGCTCGTGTTTCTACCTGTCTCTT140.17918853193395623No Hit
AGCAGAAGCGGAGCGGGGGGAGCAGAAGCTGTCTCTTATACACATCTGAC130.16638935108153077No Hit
GAAGCGGAGCGGGGGGAGCAGAAGCACGCTCGTGTTTCTGTCTCTTATAC130.16638935108153077No Hit
GAAGCGGAGCGGGGGGAGCAGAAGCAGGCTCGTGTTTCTACCTGTCTCTT120.15359017022910534No Hit
GTAGAAACACGAGCTCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATT120.15359017022910534No Hit
GAAGCGGAGCGGGGGGAGCAGAAGCACGCACGTGTTTCTACCTGTCTCTT120.15359017022910534No Hit
TATTAGTAGTAACAAGAGCCGGGTTATTAGTAGAAACAACGCACCGGGTT110.1407909893766799No Hit
CTTGTTACTACTAATAACCCGGAGTAACAAGAGCCCGGGTTATTAGTAGA110.1407909893766799No Hit
AAACACGAGCGTGCTTCTGCTCCCCCCGCTCCGCTTCTGCTCCTGTCTCT100.12799180852425446No Hit
GTAGAAACACGAGCCTGCTTCTGCTCCCCCCGCTCCGCTTCCTGTCTCTT100.12799180852425446No Hit
GAAGCGGAGCGGGGGGAGCAGAAGCAGGCACGTGTTTCTACCTGTCTCTT100.12799180852425446No Hit
ATTAGTAGTAACAAGAGCTCCGGGTTATTAGTAGTAACAAGAGCCCGGGT90.115192627671829No Hit
AACAAGAGCTCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAG90.115192627671829No Hit
CTAATAACCCGGTGCGTTGTTTCTACTAATAACCCGGCTCTTGTTACTAC90.115192627671829No Hit
GAAGCGGAGCGGGGGGAGCAGAAGCTCGTGTTTCTACCTGTCTCTTATAC80.10239344681940356No Hit
AGCAGAAGCGGAGCGGGGGGAGCAGAAGCACGCTCGTGTTTCTACCTGTC80.10239344681940356No Hit
AAACACGAGCGTGCTTCTGCTCCCCCCGCTCCGCTTCCTGTCTCTTATAC80.10239344681940356No Hit
GTAGAAACACGTGCGTGCTTCTGCTCCCCCCGCTCCGCTTCTGCTCCTGT80.10239344681940356No Hit
GTAGAAACACGTGCGTGCTTCTGCTCCCCCCAAGTGCGTGCTTCTGCTCC80.10239344681940356No Hit
TGTTTCTACTAATAACCCGGGCTCTTGTTACTACTAATAACCCGGAGCTC80.10239344681940356No Hit
TGTTTCTACTAATAACCCGGTGCGTTGTTTCTACTAATAACCCGGCTCTT80.10239344681940356No Hit
GTAACAAGAGCTCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGT80.10239344681940356No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTATT100.0069566313145.0145
CTATTCA100.0069566313145.0145
ACGAGCC301.5009753E-596.6666649
AGAAGCG606.9485395E-1084.5833365
GTAGTAA702.0445987E-762.1428571
ACGTGCG350.003434474662.1428579
GCGGAGC851.0935764E-859.7058839
AGCGGAG851.0935764E-859.7058838
AAGCGGA851.0935764E-859.7058837
GAAACAC1251.8189894E-1258.0000044
TAGTAAC753.294972E-758.0000042
AACACGT501.8878386E-458.06
GAAGCGG901.7176717E-856.388896
ACAAGAG805.146976E-754.3757
AAACACG1403.6379788E-1251.7857135
CAAGAGC857.822655E-751.176478
ACACGTG604.6456535E-448.3333327
AACAAGA901.1604316E-648.3333326
AGTAACA901.1604316E-648.3333323
GTAACAA901.1604316E-648.3333324
Produced by FastQC (version 0.11.5)