FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_DEMUX_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_DEMUX_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5864
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGACAACCATGCACCACCTGTCTCCTCTGTCCCGAAGGCCGCACCTAT1031.7564802182810368No Hit
GTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACA631.0743519781718964No Hit
ACCATGCACCACCTGTCTCCTCTGTCCCGAAGGCCGCACCTATCTCTAGG621.0572987721691678No Hit
GTAGAAACACGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCC591.0061391541609823No Hit
GTAGTAACAAGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCC460.7844474761255117No Hit
ATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCTCTGAATC390.665075034106412No Hit
GCAGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGG390.665075034106412No Hit
GTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCTCT370.6309686221009551No Hit
ATCTCTAGGTGATTCAGAGGGATGTCAAGACCTGGTAAGGTTCTTCGCGT320.5457025920873124No Hit
GTAGAAACACGTGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCC320.5457025920873124No Hit
GAACCTTACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCG270.46043656207366984No Hit
GCGGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGG250.42633015006821284No Hit
GTCCCGAAGGCCGCACCTATCTCTAGGTGATTCAGAGGGATGTCAAGACC240.4092769440654843No Hit
ATTAGTAGAAACACGAGCTGACGACAACCATGCACCACCTGTCTCCTCTG240.4092769440654843No Hit
CCTGTCTCCTCTGTCCCGAAGGCCGCACCTATCTCTAGGTGATTCAGAGG230.39222373806275584No Hit
GGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTG220.3751705320600273No Hit
AAGCAGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCA210.3581173260572988No Hit
CCTAGAGATAGGTGCGGCCTTCGGGACAGAGGAGACAGGTGGTGCATGGT200.34106412005457026No Hit
CCTCTGTCCCGAAGGCCGCACCTATCTCTAGGTGATTCAGAGGGATGTCA190.32401091405184174No Hit
TATTAGTAGTAACAAGAGCTGACGACAACCATGCACCACCTGTCTCCTCT180.30695770804911326No Hit
ACCTTACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGGC180.30695770804911326No Hit
AAGCGGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCA170.2899045020463847No Hit
GTCTCCTCTGTCCCGAAGGCCGCACCTATCTCTAGGTGATTCAGAGGGAT170.2899045020463847No Hit
AACAAGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCCGAAGG160.2728512960436562No Hit
GTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGGCCTTCGGGACA160.2728512960436562No Hit
TACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGGCCTTC160.2728512960436562No Hit
ATTCAGAGGGATGTCAAGACCTGGTAAGGTTCTTCGCGTTGCTTCGAATT150.25579809004092774No Hit
CGAAGAACCTTACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAGG150.25579809004092774No Hit
GGTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGGCCTTCGGGAC150.25579809004092774No Hit
GTCAAGACCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATACT140.23874488403819918No Hit
CGACAACCATGCACCACCTGTCTCCTCTGTCCCGAAGGCCGCACCTATCT140.23874488403819918No Hit
ATTAGTAGTAACAAGAGCTGACGACAACCATGCACCACCTGTCTCCTCTG140.23874488403819918No Hit
TGTCTCCTCTGTCCCGAAGGCCGCACCTATCTCTAGGTGATTCAGAGGGA140.23874488403819918No Hit
ACCTAGAGATAGGTGCGGCCTTCGGGACAGAGGAGACAGGTGGTGCATGG140.23874488403819918No Hit
GCGAAGAACCTTACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAG130.22169167803547069No Hit
CCTCTGAATCACCTAGAGATAGGTGCGGCCTTCGGGACAGAGGAGACAGG130.22169167803547069No Hit
AGTAACAAGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCCGA130.22169167803547069No Hit
ATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATC130.22169167803547069No Hit
GTAGAAACACGAGCTGACGACAACCATGCACCACCTGTATCCATGTCCCC120.20463847203274216No Hit
TCTCTAGGTGATTCAGAGGGATGTCAAGACCTGGTAAGGTTCTTCGCGTT120.20463847203274216No Hit
GAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGA120.20463847203274216No Hit
TGCACCACCTGTCTCCTCTGTCCCGAAGGCCGCACCTATCTCTAGGTGAT120.20463847203274216No Hit
GAGCAGAAGCGGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACC120.20463847203274216No Hit
CTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGGCCTTCGGGACAGA120.20463847203274216No Hit
AGCAGAAGCAGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCT120.20463847203274216No Hit
AAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCTCTGAATCACCTA110.18758526603001366No Hit
AGAGATAGGTGCGGCCTTCGGGACAGAGGAGACAGGTGGTGCATGGTTGT110.18758526603001366No Hit
ATTAGTAGAAACACGTGCTGACGACAACCATGCACCACCTGTCTCCTCTG110.18758526603001366No Hit
AACACGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCCGAAGG110.18758526603001366No Hit
GGATTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTG100.17053206002728513No Hit
GACGACAACCATGCACCACCTGTATCCATGTCCCCGAAGGGAACGTCTAA100.17053206002728513No Hit
GCAGAAGCAGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTT100.17053206002728513No Hit
GCAGAGGATTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGG100.17053206002728513No Hit
CCTTACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGGCC100.17053206002728513No Hit
TATTAGTAGAAACACGAGCTGACGACAACCATGCACCACCTGTCTCCTCT90.15347885402455663No Hit
CCTATCTCTAGGTGATTCAGAGGGATGTCAAGACCTGGTAAGGTTCTTCG90.15347885402455663No Hit
CCTGTATCCATGTCCCCGAAGGGAACGTCTAATCTCTTAGATTTGCATAG90.15347885402455663No Hit
GCGGTGGAGCATGTGGTTTAATTCGAAGCTACGCGAAGAACCTTACCAGG90.15347885402455663No Hit
ACCATGCACCACCTGTATCCATGTCCCCGAAGGGAACGTCTAATCTCTTA90.15347885402455663No Hit
AGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGAC90.15347885402455663No Hit
AGTAGAAACACGTGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCC80.1364256480218281No Hit
CACCTAGAGATAGGTGCGGCCTTCGGGACAGAGGAGACAGGTGGTGCATG80.1364256480218281No Hit
ACGCGAAGAACCTTACCAGGTCTTGACATCCCTCTGAATCACCTAGAGAT80.1364256480218281No Hit
CTCTAGGTGATTCAGAGGGATGTCAAGACCTGGTAAGGTTCTTCGCGTTG80.1364256480218281No Hit
CTGTCTCCTCTGTCCCGAAGGCCGCACCTATCTCTAGGTGATTCAGAGGG80.1364256480218281No Hit
ATTCGAAGCTACGCGAAGAACCTTACCAGGTCTTGACATACTATGCAAAT80.1364256480218281No Hit
AGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCCGAAGGCCGCAC80.1364256480218281No Hit
CCATGCACCACCTGTCTCCTCTGTCCCGAAGGCCGCACCTATCTCTAGGT80.1364256480218281No Hit
GGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCTC70.11937244201909959No Hit
TAACAAGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCCGAAG70.11937244201909959No Hit
GAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCCGAAGGCCGCA70.11937244201909959No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTA70.11937244201909959No Hit
GAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCT70.11937244201909959No Hit
TTACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGGCCTT60.10231923601637108No Hit
GGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATACTCCTCTGCTTC60.10231923601637108No Hit
AGCAGAAGCAGAGGATTATGTGGTTTAATTCGAAGCAACGCGAAGAACCT60.10231923601637108No Hit
AAACACGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCCGAAG60.10231923601637108No Hit
AGCAGAAGCGGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCT60.10231923601637108No Hit
GGCCGCACCTATCTCTAGGTGATTCAGAGGGATGTCAAGACCTGGTAAGG60.10231923601637108No Hit
TTAGTAGAAACACGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGT60.10231923601637108No Hit
CCCGAAGGCCGCACCTATCTCTAGGTGATTCAGAGGGATGTCAAGACCTG60.10231923601637108No Hit
CTTCGGGACAGAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTTT60.10231923601637108No Hit
CAGAAGCGGAGCCTGAGCAGCCTGAGGAGCGAGGACACCGCCGTGTTTCT60.10231923601637108No Hit
AGTAGAAACACGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCC60.10231923601637108No Hit
CTTCTGCTCCCCCCGCTCCGCTTCTGCTCTGTCTCTTATACACATCTGAC60.10231923601637108No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGAGG100.006912936145.08
TAGTAAC502.0321659E-672.52
TAACAAG502.0321659E-672.55
ACAAGAG502.0321659E-672.57
AACAAGA553.5799876E-665.909096
AGTAACA553.5799876E-665.909093
GTAACAA553.5799876E-665.909094
GTAGTAA451.0977761E-464.444441
AAGAGCT350.003391895462.1428579
TAGAAAC659.645353E-655.769232
GTAGAAA659.645353E-655.769231
CAAGAGC400.005755712354.3758
GAAACAC701.4962106E-551.7857134
GCAGAAG450.00917061448.3333321
AGAAACA803.29669E-545.31253
AACACGA709.7276916E-441.428576
AAACACG959.0903704E-538.157895
TGACGAC550.002554024815.81818215-19
ACAACCA600.004558929714.50000120-24
ACCACCT650.007750354713.38461530-34
Produced by FastQC (version 0.11.5)