FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_99_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_99_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1600323
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA20050.080.9970861
CGGAGCG502.3095163E-672.486035
TAGTAAC22950.070.439652
AAACACG21000.066.963295
CGACGAA4600.066.201515145
AACACGT9200.063.8112456
ACACGAG13200.063.1428157
ACACGTG9100.062.9195637
ACGAGCC5700.062.304769
CACGAGC13750.059.562898
AACACGA14350.058.5876546
AGTAACA30550.053.390793
GCCGACC1500.053.17138145
GTAACAA30550.053.148514
CAACGCA7050.051.4021039
ACAACGC5700.050.8610278
TAACAAG33700.048.82595
TGACGCC3300.048.33762145
ACGAGCT2850.048.3179789
ACGAGCA6750.047.2442449
Produced by FastQC (version 0.11.5)