FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_95_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_95_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003830
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA30550.15245804284794617No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG22880.11418134272867458No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA22870.1141314382956638No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGA259.380669E-487.01742145
TAGTAAC44900.082.337362
GTAGTAA47400.078.918161
AAACACG32350.075.730725
GCACGCA11200.071.194712
GTAACAA51900.070.526724
CGACGAA5700.069.970146145
ACACGAG24000.069.7516567
AACACGA25100.066.983536
ACGAGCG1450.064.974059
CGCGATA451.1966587E-464.4171148
AGTAACA58050.063.5559353
CGCACTT12450.063.4580655
TGACGCC4250.063.130283145
CACGAGC26250.062.9447258
AGCACGC12900.061.2551231
TAACAAG60700.060.8991435
GAAACAC40600.060.342094
ACGAGCC9600.058.8827369
AACACGT13300.058.302336
Produced by FastQC (version 0.11.5)