FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_92_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_92_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1987822
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG33060.16631267789570697No Hit
TTCTTAATCTATGGGCCATAGCTTCAAGCATGCTGTGCTGACCTACTGGT31690.15942071271975056No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT30360.15272997280440603No Hit
GTATTGGGATAGAAATGGTACATGATGGTGGGAAAACGACTTGGCACTCA28440.1430711602950365No Hit
CCCATACACTGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA26630.13396571725234957No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACT25040.1259670131430279No Hit
CTTCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACA24620.1238541479066033No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT24010.12078546268227235No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA23630.11887382270645963No Hit
CTGTAGGACATTTGTCCATTGAAGGCATCAAAGAAGCAGATATAACCCCA23000.11570452485182275No Hit
GTACATGATGGTGGGAAAACGACTTGGCACTCAGCAGCAACAGCCATTTA21760.1094665417728549No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA21570.10851072178494856No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAG12450.078.574367
ACGAGCG950.076.282649
ACAACGC8350.074.6325768
CAACGCA8800.072.46859
TGACGCC7600.069.65349145
CACGAGC14500.065.966358
TTATCCG350.00357174362.122114
AAACACG22550.060.7431565
TAGTAAC21500.060.6789362
AACACGA16550.059.982996
TCTCGGG12300.058.95606145
ACGAGCC11850.057.4855659
GCCGACC3800.057.249443145
GTAGTAA24700.052.834991
GAAACAC27500.050.864834
TAACAAG25600.050.675165
AGTAACA25750.050.38253
TAGAAAC31800.049.9138342
GTAACAA25800.049.7217674
ATTAGTA12750.048.334231
Produced by FastQC (version 0.11.5)