FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_8_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_8_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3656
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGAAACAACGCACTTCCGGGTTATTAGTAGAAACACCGGGTTATTAG70.19146608315098468No Hit
AGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAG70.19146608315098468No Hit
AGTAGAAACAACGCACTTCCGGGTTATTAGTAGAAACACGACCGGGTTAT70.19146608315098468No Hit
GCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGC60.16411378555798686No Hit
GCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGC50.13676148796498905No Hit
GTAGAAACACGAGCTGACGACAGCCATGCAGCACCTGTGTTACCGTTCTC50.13676148796498905No Hit
GGTAAAAGGTGTTGTCGATAGCTTTGGGATAGATTACATTGCTAAAGCTG40.10940919037199125No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA3700.0073746895.0945945110-114
Produced by FastQC (version 0.11.5)