FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_86_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_86_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3440354
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG49180.14295040568499637No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT46750.13588717905192316No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT46060.13388157148944557No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC43810.12734154682919258No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGAGGGT42820.12446393597868126No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC40280.11708097480666232No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA39500.11481376625777463No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC39000.11336042744438507No Hit
CTTTACATATAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGGG38460.11179082152592437No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA38340.11144202021071087No Hit
GTATTGGGATAGAAATGGTACATGATGGTGGGAAAACGACTTGGCACTCA37840.10998868139732133No Hit
TCCCAATACAGGGGACATCACATTTCTTATCTTTTATTTCGAAGCCAAAG36600.10638440114011524No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG35880.10429159324883427No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC35780.10400092548615636No Hit
CTATATGTGTCAGCTTCATTGTCATACTTACTATATTCGGATATATTGCT35340.10272198733037355No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA34720.10091984720177051No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCC6400.090.64445145
GCACGCA2700.080.5447162
GACGAAA12000.076.14134145
CGCGAGG300.00193449772.515564145
AGCACGC3000.072.500791
AACACGA13750.072.201446
ACAACGC7050.071.955198
GTAACAA54550.070.156554
AAACACG20250.070.155245
CACGAGC14100.069.385378
ACACGAG14550.068.235577
GAAACAC20800.067.95174
CAACGCA7400.067.5735869
ACGTGCC2750.065.882019
CACGCAC3500.062.1335953
ACGAGCC7400.061.697629
ACGTGCA3950.058.7100529
TAGTAAC67650.057.756462
GCCGACC1400.056.976517145
ACGAGCA8050.054.915329
Produced by FastQC (version 0.11.5)