FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n02_FLUB_85_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n02_FLUB_85_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1739804
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAG21310.12248506153566723No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA20240.1163349434763916No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA37650.098.193651
TAGTAAC40300.091.366492
AGCACGC14000.082.845731
AGTAACA47500.077.6698463
GTAACAA47400.077.525654
GCACGCA15700.073.8666842
AAACACG26650.072.883365
CGACGAA3850.071.57535145
TAACAAG53250.069.1408845
ACGAGCG1800.068.441159
CGCACTT16400.066.2885065
AACACGT11450.061.391346
AAGAGCG8700.059.9727639
ACGCACT21300.059.8892444
GAAACAC35100.056.9925044
TGACGCC5100.056.87618145
ACACGAG22250.055.693817
CACGCAC21200.054.7031563
CAAGAGC65600.053.245648
ACAAGAG69500.052.7602167
Produced by FastQC (version 0.11.5)